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Job ID :
47791BR
Location :
US - California - Carlsbad
:
Job Description

Scope of Need

The successful individual will directly interact with senior R&D scientists and project management to execute project workplans. The scientist will be responsible for supporting or executing these plans to meet agreed upon deliverables and timelines. These activities will require a high level of independence as well as the ability to work in a highly cooperative team.

This role will involve the following tasks:

  • Molecular biology
    • Plasmid subcloning, DNA preparations, sequencing, in vitro transcription of RNA, packaging of lentivirus, PCR, RT-PCR, TaqMan® qPCR
    • Generation of genome engineering tools
  • Tissue culture (culture of many cell types)
  • Genomic editing (TALN and CRISPR based editing, design and implementation)
  • Stable cell line generation (transfection, transduction, antibiotic-selection, clonal expansion)

This individual will also be expected to support and participate in R&D and project management meetings.

Capabilities and Competencies

This position requires the following capabilities and competencies:

  • Perform standard laboratory math calculations and solutions/media preparation
  • Proven experience:
    • Manipulating DNA plasmid constructs
    • Designing and carrying out PCR
    • Sterile tissue culture technique
    • Transfecting mammalian cells
    • Selecting stable cell populations and cloning cell lines
  • Ability to:
    • Observe deviations from the expected outcomes and trouble-shoot
    • Succeed with a moderate level of supervision and guidance
  • Strong teamwork orientation with demonstrable influence/interpersonal skills

Education:

Candidates with a bachelor’s degree or a master’s degree will be considered.


Experience:

Required: 1-5 years experience in molecular biology techniques (DNA/RNA)

Required: 1-5 years experience in stable cell line generation

Preferred: 1+ year experience in industry

Contacts:

The Program Manager will interact with internal employees and management, as well as third party providers, and customers.

Working Conditions:

The vast majority of working time will be spent in a laboratory environment. Work will take place in biosafety hoods in a tissue culture laboratory, working with various cell cultures. Cells will typically be validated by molecular biology techniques or functional assays. Time may be spent manipulating plasmid DNA constructs in a laboratory environment. Some time will be spent at the desk recording laboratory activities appropriately in notebooks.

Must be capable of observing and adhering to lab safety standards and protocols. Occasional weekend work may be required to maintain cell cultures properly.

Critical to success in this position will be the individual’s ability to work independently on some projects and to provide support collaboratively on others. This individual must be able to identify deviations from expected outcomes and participate in trouble-shooting those deviations and suggesting alternatives. Equally important will be the ability to work as part of a team in the laboratory (extended R&D team) as well as a broader cross-functional team (R&D, Project Management, Collaborator).

*LSG-IND




Thermo Fisher Scientific is an EEO/Affirmative Action Employer and does not discriminate on the basis of race, color, religion, sex, sexual orientation, gender identity, national origin, protected veteran status, disability or any other legally protected status.
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